Liquid chromatography method for simultaneous quantification of ATP and its degradation products compatible with both UV–Vis and mass spectrometry
AS Law, PS Hafen, JJ Brault - Journal of Chromatography B, 2022 - Elsevier
ATP and its degradation products are essential metabolic and signaling molecules.
Traditionally, they have been quantified via high-performance liquid chromatography
(HPLC) with UV–Vis detection while utilizing phosphate buffer mobile phase, but this
approach is incompatible with modern mass detection. The goal of this study was to develop
an ultra-performance liquid chromatography (UPLC) method free of phosphate buffer, to
allow for analysis of adenine nucleotides with UV–Vis and mass spectrometry (MS) …
Traditionally, they have been quantified via high-performance liquid chromatography
(HPLC) with UV–Vis detection while utilizing phosphate buffer mobile phase, but this
approach is incompatible with modern mass detection. The goal of this study was to develop
an ultra-performance liquid chromatography (UPLC) method free of phosphate buffer, to
allow for analysis of adenine nucleotides with UV–Vis and mass spectrometry (MS) …
